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Activity was monitored using fluorescence detection and quantification of the nicotinamide mononucleotide (NMN) reaction product

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succinate oxidation) have about the energy of NADH electrons: they generate a PMF that is about as great as NADH electrons and lead to the synthesis of only two (precise measurements demonstrate that it is approximately 1.5) moles of ATP per mole of FADH 2 oxidized

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